| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
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| Autores: |
Morais,Glaucia Almeida de
Takaki,Massanori
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| Data: |
1998-06-01
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| Ano: |
1998
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| Palavras-chave: |
Α-amylase
Β-amylase
Determination
Selective inactivation
Phaseolus vulgaris
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| Resumo: |
Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89131998000100003
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.41 n.1 1998
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| Direitos: |
info:eu-repo/semantics/openAccess
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